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Native MS: A Structural Biology Tool

This is a past event.

Friday, April 19 at 11:00am to 12:00pm

MMC Campus, PG5 134

Join us for a talk on "Native MS: A Structural Biology Tool” with Dr. Vicki Wysocki, Professor, Department of Chemistry and Biochemistry, NIH Resource for Mass Spectrometry Guided Structural Biology, The Ohio State University.

Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease.

Tools to address structural biology problems continue to improve.  Native mass spectrometry and associated technologies are becoming an increasingly important component of the structural biology toolbox. When the mass spectrometry approach is used early or mid-course in a structural characterization project, it can provide answers quickly using small sample amounts and samples that are not fully purified. Integration of sample preparation or purification with effective dissociation methods, ion mobility, and computational approaches provide a MS workflow that can be enabling in biochemical, synthetic biology, and systems biology approaches.  Beyond what MS can provide as a stand-alone tool, MS can also guide and/or be integrated with other structural biology approaches such as NMR, X-ray crystallography, and cryoEM. 

MS can determine whether the complex of interest exists in a single or in multiple oligomeric states and can provide characterization of topology/intersubunit connectivity, and other structural features. Examples will be presented to illustrate the role MS and surface-induced dissociation can play in guiding a structural biology workflow and will include designed protein complexes and isolated or recombinant protein and nucleoprotein complexes.